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Glucose Consuming Elevates Insulin Response In Humans During An Oral                Introduction :

The oral glucose tolerance test (OGTT) is widely used procedure that was originally developed to classify carbohydrate tolerance (1). However, because plasma glucose and insulin responses during this test reflect the ability of pancreatic β-cells to secrete insulin and sensitivity of tissues to insulin (2), the OGTT has also been often used to evaluate β-cells function and insulin resistance (3-5). In epidemiological studies, for example, fasting plasma insulin concentrations have been used as an index of insulin resistance, and the 30-min ratio of changes in plasma insulin and glucose have been used as an index of β-cells (6,7)

Although such approaches are simple, they have not been fully validated. On the other hand, hyperglycemic and euglycemic-hyperisulinemic clamp studies are well established for assessing β-cells function and insulin sensitivity (8,9), but these are complicated procedures, and they are generally impractical for use outside of specialized research centers. Thus, for epidemiological studies, screening of high-risk populations, and large-scale intervention trials, simpler methods are desirable.

In the present experiment, we are three staff in group and we studied two healthy nondiabetic volunteers ( Male and Female) and used multiple linear regression analysis to determine whether plasma glucose and insulin responses during the OGTT in addition to demographic data ( e.g., age, sex, BMI) could be used to predict β-cell function and insulin sensitivity as measured by the hyperglycemic and euglycemic-hyperinsulinemic clamp procedures. 
When blood glucose concentrations measured in the fasting state and 2 h after an OGTT, intermediate measures during an OGTT may provide additional information regarding a person's risk of future diabetes type 2, and we aimed to characterise heterogeneity of glycaemic patterns based on five time points during an OGTT.
In addition to the importance of  OGTT, Gestational diabetes occurs when a pregnant woman who doesn’t have diabetes before pregnancy has high blood sugar levels as a result of the pregnancy. The American Diabetes Association estimates that gestational diabetes occurs in 9% of pregnancies.
 
Method : ( as per protocol )
 

Results:

In our experiment in the Laboratory we select Male and Female in our group, to do Oral 

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